Детальніше використання оптичні мікроскопи і кроки використання масло занурення лінзи!
(1) Доступ до мікроскопа
Hold the mirror arm with one hand, and support the mirror base with the other hand, and gentlely place the microscope on the table, about one thumb away from the edge of the table.
Adjust the chair to an appropriate height so that you can use the microscope with ease.
Take 95 відсоток спирт і лінза очищення папір, and wipe all lenses clean (※Note: Wipe only in a single straight line).
Place the slide specimen on the stage stably, and clamp it with a slide clip (※ Note: Please confirm hether it is clamped well, so as not to cause the slide to jump up, causing injury to the students or damage to the microscope lens).
(2) використання з низьке збільшення об'єктив
Lower the stage to the lowest point first, and turn the rotating disk (Revolving nosepiece) so that the the low power lens is directly below the lens barrel.
Turn on the light source, adjust the light source adjustment knob and the aperture (Iris diaphragm), so that the brightness in the field of view is appropriate.
Place the slides to be observation on the stage, clamp them with slide clamps, and turn the control knobs (mechanical stage control knobs) to adjust the position of the slides. turn the control wheel (Регулювання ручка) доти the відстані between the low power mirror and the stage is about 0.1 cm (or the stage can no longer be raised).
Спостерігайте наскрізь окуляр, і at the same time slowly turn the adjust wheel (Adjust knob) toward yourself to lower the stage until the image is clear. If the field of view is too bright or too dark, you can adjust the light source adjust knob and the aperture (Iris diaphragm) to make the brightness in the field of view appropriate.
(3) use of high magnification lenses
First use the low-power lens to find the target to be observation, and adjust the part to the center of the field of view. Repeat A.~D. of the "Use of low-magnification lens" method.
(4) Use of oil mirror
First use the high-power mirror to find the target to be observation, and adjust the part to the center of the field of view.
Turn the high-magnification lens away, add a small drop of oil immersion oil on the glass slide of the object image range (that is, the area illuminated by the light), and use a 100X objective lens (ie oil immersion objective), carefully immerse the oil immersion lens in the oil, readjust the aperture, and slowly turn the adjustment knob (Adjustment knob) until the image is clear.
(5) Запобіжні заходи
If it is need to move the microscope, be sure to lift the microscope and put it in an appropriate position. it is strictly forbidden to push the microscope (the vibration caused by the push may cause the internal parts of the microscope to loosen, remember!!) , і будь ласка ручка мікроскоп з обережністю.
Коли використовуючи a мікроскоп, the height of the chair should be appropriate. When observing, you should be used to obething with both eyes at the same time, and the aperture and brightness of the light source should be appropriate. Інакше, you will easily feel tired when observing for a long time.
For specimen staining or any other operation, the slide should be removed, and then put back on the stage for observation after the operation is completed. Do not operate on the stage to prevent dye or other liquids from flowing into the microscope or damaging the lens.
Після спостереження один матеріал, коли ви хочете to change other material, be sure to lower the stage to the lowest point, and then refocus according to the standard procedure after changing the slide. Do not directly replace the specimen, so as not to scratch the lens or slide specimen.
До і після мікроскоп це використовуй, all лінзи повинен бути очищений з лінз папір (лінза папір) і 95 відсоток спирт. Коли витирання, вони повинні бути витираються легковажно в a одинарний прямий лінія, і повинен не бути повернутий і натертий.
After using the microscope, lower the stage to the lowest point, align the low-magnification lens with the round hole in the center of the stage, roll up the power cord, cover it with a dust cover, and store it in a storage cabinet.
If not used for a long time, all lenses should be cleaned with Xylene (Xylol).
4. Збільшення & Мікроскопічні Вимірювання
The approximate value of the magnification of the microscope is close to the product of the magnification of the eyepiece and the objective lens, but due to the difference in production, each lens has a slightly different error. To know the actual size of the observed object, first install an ocular micrometer (Ocular micrometer) between the two lenses of the eyepiece (as shown in Figure {{0}}a). Put a stage micrometer (Stage micrometer) on the stage (as shown in Figure 2-1b). The micrometer is a straight line with 1 mm divided into 100 small divisions engraved on the glass slide, that is, the length of each small division on the stage micrometer is 0.01 mm (10mm). The method of use is: move the micrometer on the stage so that the scales at one end of the two micrometers overlap to form a straight line, and then check the overlap of the scales at the other end, and calculate the number of divisions on the two micrometers in this interval.
